Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat
Material type:
ArticleLanguage: English Publication details: Dordrecht (Netherlands) : Springer, 2012.ISSN: - 1573-5036 (Online)
- 0032-079X
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
| Article | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-6835 (Browse shelf(Opens below)) | Available |
Peer review
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0032-079X
Background and aims: Crop biofortification programs require fast, accurate and inexpensive methods of identifying nutrient dense genotypes. This study investigated energy-dispersive X-ray fluorescence spectrometry (EDXRF) for the measurement of zinc (Zn), iron (Fe) and selenium (Se) concentrations in whole grain wheat. Methods: Grain samples were obtained from existing biofortification programs. Reference Zn, Fe and Se concentrations were obtained using inductively coupled plasma optical emission spectrometry (ICP-OES) and/or inductively coupled plasma mass spectrometry (ICP-MS). One set of 25 samples was used to calibrate for Zn (19–60 mg kg–1) and Fe (26–41 mg kg–1), with 25 further samples used to calibrate for Se (2–31 mg kg–1 ). Calibrations were validated using an additional 40–50 wheat samples. Results: EDXRF limits of quantification (LOQ) were estimated as 7, 3 and 2 mg kg–1 for Zn, Fe, and Se, respectively. EDXRF results were highly correlated with ICP-OES or -MS values. Standard errors of EDXRF predictions were ±2.2 mg Zn kg–1, ±2.6 mg Fe kg–1, and ±1.5 mg Se kg–1. Conclusion: EDXRF offers a fast and economical method for the assessment of Zn, Fe and Se concentration in wheat biofortification programs.
Conservation Agriculture Program|Global Wheat Program
Text in English
CIMMYT Informa No. 1812|Springer
INT2983|INT1421
CIMMYT Staff Publications Collection
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