TY  - JA
AU  - Siyang Liu
AU  - Lina Xie
AU  - Haibin Wu
AU  - Dengan Xu
AU  - Rui Che
AU  - Wenfei Tian
AU  - Bingyan Liu
AU  - Yuheng Chao
AU  - Yan Zhang
AU  - Xianchun Xia
AU  - He Zhonghu
AU  - Shuanghe Cao
TI  - TaIAA10-6D orchestrates processing quality and grain yield by modulating glutenin/gliadin ratio and plant morphogenesis in wheat
SN  - 2095-5421
PY  - 2025///
CY  - China
PB  - Elsevier B.V., KeAi Communications Co. Ltd.
KW  - Agronomic characters
KW  - AGROVOC
KW  - Auxins
KW  - Transcription factors
KW  - Processing quality
KW  - Seed storage proteins
KW  - Triticum aestivum
N1  - Peer review; Open Access
N2  - High molecular weight glutenin subunits (HMW-GS), major components of seed storage proteins in wheat, have large effects on processing quality. GLU-1 genes encode HMW-GS and their expression is mainly controlled at the transcriptional level by interactions between cis-regulatory elements and transcription factors. We previously identified an Aux/IAA transcription factor TaIAA10-6D that bound to a conserved cis-regulatory module CCRM1-1, the most essential conserved cis-regulatory module in GLU-1. Here, we confirmed the binding of TaIAA10-6D to CCRM1-1 using yeast one hybrid and dual-luciferase reporter assays. The enhanced expression of TaIAA10-6D suppressed glutenin accumulation and increased gliadin content. Dynamic transcriptome analyses revealed that TaIAA10-6D overexpression down-regulated glutenin and gliadin genes during an early stage of grain filling, but up-regulated gliadin genes during a late stage probably by endoplasmic reticulum stress, accounting for its effect on the trade-off between glutenin and gliadin. Rheological property and processing quality assays showed that TaIAA10-6D overproduction reduced stabilization time and bread quality, but enhanced cookie quality. Overexpression of TaIAA10-6D also reduced plant height, leaf size, kernel number and grain yield. We identified two major haplotypes of TaIAA10-6D, Hap I and Hap II, and developed a breeding-friendly diagnostic marker. Hap I conferred higher expression of TaIAA10-6D and concomitantly reduced plant height and kernel number, but had little effect on grain yield, contributing to lodging resistance without yield penalty. Hap I was subjected to positive selection in breeding. The findings provide a useful gene for wheat improvement and broaden insights into the regulatory machinery underpinning auxin-mediated quality formation, plant morphogenesis and yield gain
UR  - https://hdl.handle.net/10883/36188
DO  - https://doi.org/10.1016/j.cj.2025.06.002
T2  - Crop Journal
ER  -