TY  - JA
AU  - Esen,A.
TI  - Estimation of protein quality and quantity in corn (Zea mays L.) by assaying protein in two solubility fractions
SN  - 0021-8561
PY  - 1980///
CY  - United States of America
PB  - American Chemical Society
KW  - Approximation
KW  - AGROVOC
KW  - Protein quality
KW  - Zea mays
KW  - Solubilization
KW  - Zein
KW  - Polyacrylamide gel electrophoresis
KW  - Germplasm
N1  - Peer review
N2  - The protein from maize seeds can be recovered in two solubility fractions. Fraction 1 includes zeins (low quality proteins), extracted with 60% tert-butyl alcohol, and fraction 2, called nonzein (high quality proteins), includes albumins, globulins, glutelins, and others, extracted with a high pH buffer containing an ionic detergent (sodium dodecyl sulfate) and a reducing agent (2-mercaptoethanol). Protein content of the two fractions, determined by a simple Coomassie Blue R-250 dye-binding method, using comparable proteins as standards, gives an estimate of protein quantity of the sample. The zein as percent of total extractable protein or the ratio of zein to nonzein can be used to estimate the protein quality. The method offers promise as a mass-screening tool to identify the maize germ plasm with high protein and/or high quality protein
DO  - https://doi.org/10.1021/jf60229a023
T2  - Journal of Agricultural and Food Chemistry
ER  -