TY  - JA
AU  - Sodek,L.
AU  - Wilson,C.M.
TI  - Amino acid compositions of proteins isolated from normal, opaque-2, and floury-2 corn endosperms by a modified Osborne procedure
SN  - 0021-8561
PY  - 1971///
CY  - United States of America
PB  - American Chemical Society
KW  - Amino acids
KW  - AGROVOC
KW  - Protein content
KW  - Maize
KW  - Endosperm
KW  - Solvents
KW  - Propanol
KW  - Zein
KW  - Glycine (amino acid)
KW  - Methionine
KW  - Histidine
KW  - Proline
KW  - Aspartic acid
KW  - Leucine
KW  - Isoleucine
KW  - Glutelins
KW  - Polyacrylamide gel electrophoresis
N1  - Peer review
N2  - The classical Osborne-Mendel extraction procedure for corn endosperm protein was modified to extract additional protein by a solvent containing 55% 2-propanol and 0.6% 2-mercaptoethanol. The new fraction is designated zein-2, since it has an amino acid composition somewhat similar to zein, but with higher levels of glycine, methionine, histidine, and proline, and lower levels of aspartic acid, leucine, and isoleucine. Zein-2 was previously recovered as a part of the glutelin fraction. Zein-1 and zein-2 , as well as glutelin, were heterogeneous by polyacrylamide gel electrophoresis. However. these three protein fractions had characteristic amino acid compositions which were constant for normal, opaque-2, and floury-2 endosperms. The two mutations which increase the lysine content of the endosperm act by changing the proportions of proteins which contain different levels of lysine
DO  - https://doi.org/10.1021/jf60178a011
T2  - Journal of Agricultural and Food Chemistry
ER  -