TY  - JA
AU  - Mengjing Sun
AU  - Jingyang Tong
AU  - Yan Dong
AU  - Zongjun Pu
AU  - Jianmin Zheng
AU  - Yelun Zhang
AU  - Xueyong Zhang
AU  - Chenyang Hao
AU  - Xiaowan Xu
AU  - Qiang Cao
AU  - Awais Rasheed
AU  - Mohamed Badry Ali
AU  - Shuanghe Cao
AU  - Xianchun Xia
AU  - He Zhonghu
AU  - Zhongfu Ni
AU  - Yuanfeng Hao
TI  - Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring
SN  - 0040-5752
PY  - 2024///
CY  - Berlin (Germany)
PB  - Springer
KW  - Molecular characterization
KW  - AGROVOC
KW  - Quantitative Trait Loci
KW  - Wheat
KW  - Zinc
KW  - Iron
KW  - Field Experimentation
KW  - China
N1  - Peer review
N2  - Wheat is a major source of dietary energy for the growing world population. Developing cultivars with enriched zinc and iron can potentially alleviate human micronutrient deficiency. In this study, a recombinant inbred line (RIL) population with 245 lines derived from cross Zhou 8425B/Chinese Spring was used to detect quantitative trait loci (QTL) for grain zinc concentration (GZnC) and grain iron concentration (GFeC) across four environments. Three stable QTL for GZnC with all favorable alleles from Chinese Spring were identified on chromosomes 3BL, 5AL, and 5BL. These QTL explaining maxima of 8.7%, 5.8%, and 7.1% of phenotypic variances were validated in 125 resequenced wheat accessions encompassing both landraces and modern cultivars using six kompetitive allele specific PCR (KASP) assays. The frequencies of favorable alleles for QGZnCzc.caas-3BL, QGZnCzc.caas-5AL and QGZnCzc.caas-5BL were higher in landraces (90.4%, 68.0%, and 100.0%, respectively) compared to modern cultivars (45.9%, 35.4%, and 40.9%), suggesting they were not selected in breeding programs. Candidate gene association studies on GZnC in the cultivar panel further delimited the QTL into 8.5 Mb, 4.1 Mb, and 47.8 Mb regions containing 46, 4, and 199 candidate genes, respectively. The 5BL QTL located in a region where recombination was suppressed. Two stable and three less stable QTL for GFeC with favorable alleles also from Chinese Spring were identified on chromosomes 4BS (Rht-B1a), 4DS (Rht-D1a), 1DS, 3AS, and 6DS. This study sheds light on the genetic basis of GZnC and GFeC in Chinese Spring and provides useful molecular markers for wheat biofortification
DO  - https://doi.org/10.1007/s00122-024-04661-6
T2  - Theoretical and Applied Genetics
ER  -