TY  - JA
AU  - Xiaowan Xu
AU  - Daojie Sun
AU  - Zhongqiu Ni
AU  - Xinyu Zou
AU  - Xiaoting Xu
AU  - Mengjing Sun
AU  - Qiang Cao
AU  - Jingyang Tong
AU  - Fugong Ding
AU  - Yelun Zhang
AU  - Fengju Wang
AU  - Yachao Dong
AU  - Luyan Zhang
AU  - Jiankang Wang
AU  - Xianchun Xia
AU  - He Zhonghu
AU  - Yuanfeng Hao
TI  - Molecular identification and validation of four stable QTL for adult-plant resistance to powdery mildew in Chinese wheat cultivar Bainong 64
SN  - 0040-5752
PY  - 2023///
CY  - Berlin (Germany)
PB  - Springer Verlag, 
KW  - AGROVOC
KW  - Quantitative Trait Loci
KW  - Powdery mildews
KW  - Wheat
KW  - Disease resistance
KW  - Genotyping
N1  - Peer review
N2  - Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating fungal disease. Seedlings of Chinese wheat Bainong 64 are susceptible to Bgt, but adult plants have maintained resistance since it was released in 1996. A population of 171 recombinant inbred lines (RILs) developed from cross Jingshuang 16/Bainong 64 (JS16/BN64) was used to dissect genetic components of powdery mildew resistance. A genetic map comprising 5383 polymorphic markers was constructed using the 15 K SNP chip and kompetitive allele-specific PCR (KASP) markers. Composite interval mapping identified four stable QTL with favorable alleles all from BN64 on chromosome arms 1DL, 2BS, 2DL, and 6BL in at least four environments. They accounted for 8.3%, 13.8%, 14.4%, and 9.0% of the total phenotypic variation explained (PVE) in maximum, respectively. QPmjbr.caas-1DL, situated about 22 Mb from centromere, is probably a new QTL. QPmjbr.caas-2DL located near the end of arm 2DL and explained the largest PVE. Using genetic maps populated with KASP markers, QPmjbr.caas-2BS and QPmjbr.caas-6BL were fine mapped to a 1.8 cM genetic intervals spanning 13.6 Mb (76.0-89.6 Mb) and 1.7 cM and 4.9 Mb (659.9-664.8 Mb), respectively. The four QTL independent of stripe rust and leaf rust resistance were validated for powdery mildew resistance in another RIL population related to BN64 and a cultivar panel using representative KASP markers. Since BN64 has been a leading cultivar and an important breeding parent in China, the QTL and markers reported in this study will be useful for marker-assisted selection of APR
T2  - Theoretical and Applied Genetics 
DO  - https://doi.org/10.1007/s00122-023-04481-0
ER  -