TY  - JA
AU  - Ramu,P.
AU  - Rakesh K. Srivastava
AU  - Sanyal,A.
AU  - Fengler,K.
AU  - Jun Cao
AU  - Yun Zhang
AU  - Nimkar,M.
AU  - Gerke,J.
AU  - Shreedharan,S.
AU  - Llaca,V.
AU  - May,G.D.
AU  - Peterson-Burch,B.
AU  - Haining Lin
AU  - King,M.
AU  - Das,S.
AU  - Bhupesh,V.
AU  - Mandaokar,A.
AU  - Maruthachalam,K.
AU  - Krishnamurthy,P.
AU  - Gandhi,H.
AU  - Rathore,A.
AU  - Gupta,R.
AU  - Annapurna Chitikineni
AU  - Bajaj,P.
AU  - Gupta,S.K.
AU  - Satyavathi,C.T.
AU  - Pandravada,A.
AU  - Varshney,R.K.
AU  - BABU,R.
TI  - Improved pearl millet genomes representing the global heterotic pool offer a framework for molecular breeding applications
PY  - 2023///
CY  - USA : 
PB  - Springer Nature
KW  - AGROVOC
KW  - Pearl millet
KW  - Genetic variation
KW  - Food security
KW  - Genomes
KW  - Hybrids
N1  - Peer review; Open Access
N2  - High-quality reference genome assemblies, representative of global heterotic patterns, offer an ideal platform to accurately characterize and utilize genetic variation in the primary gene pool of hybrid crops. Here we report three platinum grade de-novo, near gap-free, chromosome-level reference genome assemblies from the active breeding germplasm in pearl millet with a high degree of contiguity, completeness, and accuracy. An improved Tift genome (Tift23D(2)B(1)-P1-P5) assembly has a contig N50 similar to 7,000-fold (126 Mb) compared to the previous version and better alignment in centromeric regions. Comparative genome analyses of these three lines clearly demonstrate a high level of collinearity and multiple structural variations, including inversions greater than 1 Mb. Differential genes in improved Tift genome are enriched for serine O-acetyltransferase and glycerol-3-phosphate metabolic process which play an important role in improving the nutritional quality of seed protein and disease resistance in plants, respectively. Multiple marker-trait associations are identified for a range of agronomic traits, including grain yield through genome-wide association study. Improved genome assemblies and marker resources developed in this study provide a comprehensive framework/platform for future applications such as marker-assisted selection of mono/oligogenic traits as well as whole-genome prediction and haplotype-based breeding of complex traits
T2  - Communications Biology
DO  - https://doi.org/10.1038/s42003-023-05258-3
ER  -