TY  - JA
AU  - Runmiao Tian
AU  - Jianjun Jiang
AU  - Shirong Bo
AU  - Hui Zhang
AU  - Xuehai Zhang
AU  - Hearne,S.
AU  - Jihua Tang
AU  - Dong Ding
AU  - Zhiyuan Fu
TI  - Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development
SN  - 1471-2229
PY  - 2023///
CY  - London (United Kingdom)
PB  - BioMed Central
KW  - Maize
KW  - AGROVOC
KW  - Endosperm
KW  - Membranes
KW  - Proteomics
KW  - Transcriptomics
N1  - Peer review; Open Access
N2  - Background: Glycosylphosphatidylinositol (GPI) and GPI-anchored proteins (GAPs) are important for cell wall formation and reproductive development in Arabidopsis. However, monocot counterparts that function in kernel endosperm development have yet to be discovered. Here, we performed a multi-omic analysis to explore the function of GPI related genes on kernel development in maize. Results: In maize, 48 counterparts of human GPI synthesis and lipid remodeling genes were identified, in which null mutation of the glucosaminyl-phosphatidylinositol O-acyltransferase1 gene, ZmGWT1, caused a kernel mutant (named gwt1) with defects in the basal endosperm transport layer (BETL). We performed plasma membrane (PM) proteomics to characterize the potential GAPs involved in kernel development. In total, 4,981 proteins were successfully identified in 10-DAP gwt1 kernels of mutant and wild-type (WT), including 1,638 membrane-anchored proteins with different posttranslational modifications. Forty-seven of the 256 predicted GAPs were differentially accumulated between gwt1 and WT. Two predicted BETL-specific GAPs (Zm00001d018837 and Zm00001d049834), which kept similar abundance at general proteome but with significantly decreased abundance at membrane proteome in gwt1 were highlighted. Conclusions: Our results show the importance of GPI and GAPs for endosperm development and provide candidate genes for further investigation of the regulatory network in which ZmGWT1 participates
UR  - https://hdl.handle.net/10883/22580
DO  - https://doi.org/10.1186/s12870-023-04188-w
T2  - BMC Plant Biology
ER  -