TY  - JA
AU  - Khan,N.
AU  - Yanfei Zhang
AU  - Jingyi Wang
AU  - Yuying Li
AU  - Xin Chen
AU  - Lili Yang
AU  - Jie Zhang
AU  - Chaonan Li
AU  - Long Li
AU  - Shoaib-ur-Rehman
AU  - Reynolds,M.P.
AU  - Lichao Zhang
AU  - Xueyong Zhang
AU  - Xinguo Mao
AU  - Ruilian Jing
TI  - TaGSNE, a WRKY transcription factor, overcomes the trade-off between grain size and grain number in common wheat and is associated with root development
SN  - 0022-0957
PY  - 2022///
CY  - Oxford (United Kingdom)
PB  - Oxford University Press, 
KW  - Grain
KW  - AGROVOC
KW  - Yields
KW  - Haplotypes
KW  - Wheat
KW  - Roots
N1  - Peer review; Reference only
N2  - Wheat is one of the world's major staple food crops, and breeding for improvement of grain yield is a priority under the scenarios of climate change and population growth. WRKY transcription factors are multifaceted regulators in plant growth, development, and responses to environmental stimuli. In this study, we identify the WRKY gene TaGSNE (Grain Size and Number Enhancer) in common wheat, and find that it has relatively high expression in leaves and roots, and is induced by multiple abiotic stresses. Eleven single-nucleotide polymorphisms were identified in TaGSNE, forming two haplotypes in multiple germplasm collections, named as TaGSNE-Hap-1 and TaGSNE-Hap-2. In a range of different environments, TaGSNE-Hap-2 was significantly associated with increases in thousand-grain weight (TGW; 3.0%) and spikelet number per spike (4.1%), as well as with deeper roots (10.1%) and increased root dry weight (8.3%) at the mid-grain-filling stage, and these were confirmed in backcross introgression populations. Furthermore, transgenic rice lines overexpressing TaGSNE had larger panicles, more grains, increased grain size, and increased grain yield relative to the wild-type control. Analysis of geographic and temporal distributions revealed that TaGSNE-Hap-2 is positively selected in China and Pakistan, and TaGSNE-Hap-1 in Europe. Our findings demonstrate that TaGSNE overcomes the trade-off between TGW/grain size and grain number, leading us to conclude that these elite haplotypes and their functional markers could be utilized in marker-assisted selection for breeding high-yielding varieties
DO  - https://doi.org/10.1093/jxb/erac327
T2  - Journal of Experimental Botany
ER  -