TY  - JA
AU  - Wei Wang
AU  - Hui Wei
AU  - Alahuhta,M.
AU  - Xiaowen Chen
AU  - Hyman,D.
AU  - Johnson,D.K.
AU  - Min Zhang
AU  - Himmel,M.E.
TI  - Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica
SN  - 1932-6203
PY  - 2014///
CY  - San Francisco, CA (USA)
PB  - Public Library of Science 
KW  - Xylose
KW  - AGROVOC
KW  - Gene Expression
KW  - Yarrowia lipolytica
KW  - Yeasts
N1  - Peer review; Open Access
N2  - To develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose Yarrowia lipolytica as a viable demonstration strain. Y. lipolytica is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to break down biomass directly. While research is continuing on the development of a Y. lipolytica strain able to degrade cellulose, in this study, we present successful expression of several xylanases in Y. lipolytica. The XynII and XlnD expressing Yarrowia strains exhibited an ability to grow on xylan mineral plates. This was shown by Congo Red staining of halo zones on xylan mineral plates. Enzymatic activity tests further demonstrated active expression of XynII and XlnD in Y. lipolytica. Furthermore, synergistic action in converting xylan to xylose was observed when XlnD acted in concert with XynII. The successful expression of these xylanases in Yarrowia further advances us toward our goal to develop a direct microbial conversion process using this organism
UR  - https://doi.org/10.1371/journal.pone.0111443
DO  - https://doi.org/10.1371/journal.pone.0111443
T2  - PLoS ONE
ER  -