TY  - JA
AU  - Dreisigacker,S.
AU  - Yonggui Xiao
AU  - Sehgal,D.
AU  - Guzman,C.
AU  - He Zhonghu
AU  - Xianchun Xia
AU  - Peña-Bautista,R.J.
TI  - SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat
SN  - 1932-6203
PY  - 2020///
CY  - San Francisco, CA (USA)
PB  - Public Library of Science
KW  - Wheat
KW  - AGROVOC
KW  - Single nucleotide polymorphisms
KW  - Bread
KW  - Genotypes
KW  - Gluten
KW  - China
N1  - Peer review; Open Access; WC
N2  - The content and composition of seed storage proteins is largely responsible for wheat end-use quality. They mainly consist of polymeric glutenins and monomeric gliadins. According to their electrophoretic mobility, gliadins and glutenins are subdivided into several fractions. Glutenins are classified as high molecular weight or low molecular weight glutenin subunits (HMW-GSs and LMW-GSs, respectively). LMW-GSs are encoded by multigene families located at the orthologous Glu-3 loci. We designed a set of 16 single-nucleotide polymorphism (SNP) markers that are able to detect SDS-PAGE alleles at the Glu-A3 and Glu-B3 loci. The SNP markers captured the diversity of alleles in 88 international reference lines and 27 Mexican cultivars, when compared to SDS-PAGE and STS markers, however, showed a slightly larger percent of multiple alleles, mainly for Glu-B3. SNP markers were then used to determine the Glu-1 and Glu-3 allele composition in 54 CIMMYT historical lines and demonstrated to be useful tool for breeding programs to improve wheat end product properties
UR  - https://hdl.handle.net/10883/21099
DO  - https://doi.org/10.1371/journal.pone.0233056
T2  - PLoS One
ER  -