TY  - JA
AU  - Zeringue,H.J.
AU  - Shih,B.Y.
AU  - Maskos,K.
AU  - Grimm,D.
TI  - Identification of the bright-greenish-yellow-fluorescence (BGY-F) compound on cotton lint associated with aflatoxin contamination in cottonseed
SN  - 0031-9422
PY  - 1999///
CY  - United Kingdom
PB  - Elsevier
KW  - Gossypium
KW  - AGROVOC
KW  - Malvaceae
KW  - Fluorescence
KW  - Aspergillus flavus
KW  - Aflatoxins
KW  - Kojic acid
N1  - Peer review
N2  - In order to characterize the structure of the bright-greenish-yellow-fluorescence (BGY-F) compound on cotton lint associated with aflatoxin contamination in cotton seed, various in vitro and in vivo natural BGY-F reaction products were prepared. Under similar high pressure liquid chromatography separation with variable wavelength and programmable fluorescence detection (HPLC–UV/FL), combined with atmospheric pressure ionization and mass spectral determinations it was found that the BGY-F reaction products prepared from three preparations: (a) kojic acid (KA) + peroxidase (soybean peroxide or horseradish type VI and type II) + H2O2, or (b) detached fresh cotton locules + KA + H2O2, or (c) attached field cotton locules that were treated with a spore suspension of aflatoxigenic Aspergillus flavus, all resulted in identical chromatographic characteristics, and all exhibited a molecular weight of 282. Further characterization of the BGY-F reaction product with 1H- and 13C-NMR spectroscopic analysis revealed that it was a dehydrogenator dimer of 2 KA, linked through the C-6 positions
DO  - https://doi.org/10.1016/S0031-9422(99)00432-X
T2  - Phytochemistry
ER  -