TY  - JA
AU  - Brown,K.A.
AU  - Harris,D.F.
AU  - Wilker,M.B.
AU  - Rasmussen,A.
AU  - Khadka,N.
AU  - Hamby,H.
AU  - Keable,S.
AU  - Dukovic,G.
AU  - Peters,J.W.
AU  - Seefeldt,L.C.
AU  - King,P.W.
TI  - Light-driven dinitrogen reduction catalyzed by a CdS: nitrogenase MoFe protein biohybrid
SN  - 0036-8075
PY  - 2016///
CY  - Washington, DC (USA)
PB  - AAAS
KW  - AGROVOC
KW  - Nitrogen
KW  - Nitrogenase
KW  - Molybdenum
KW  - Cadmium
N1  - Peer review
N2  - The splitting of dinitrogen (N2) and reduction to ammonia (NH3) is a kinetically complex and energetically challenging multistep reaction. In the Haber-Bosch process, N2 reduction is accomplished at high temperature and pressure, whereas N2 fixation by the enzyme nitrogenase occurs under ambient conditions using chemical energy from adenosine 5′-triphosphate (ATP) hydrolysis. We show that cadmium sulfide (CdS) nanocrystals can be used to photosensitize the nitrogenase molybdenum-iron (MoFe) protein, where light harvesting replaces ATP hydrolysis to drive the enzymatic reduction of N2 into NH3. The turnover rate was 75 per minute, 63% of the ATP-coupled reaction rate for the nitrogenase complex under optimal conditions. Inhibitors of nitrogenase (i.e., acetylene, carbon monoxide, and dihydrogen) suppressed N2 reduction. The CdS:MoFe protein biohybrids provide a photochemical model for achieving light-driven N2 reduction to NH3
T2  - Science
DO  - https://doi.org/10.1126/science.aaf2091
ER  -