TY  - JA
AU  - Ellis, M.
AU  - Spielmeyer,W.
AU  - Gale,K.
AU  - Rebetzke,G.J.
AU  - Richards,R.A.
TI  - "Perfect" markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat
SN  - 0040-5752
PY  - 2002///
CY  - Berlin (Germany)
PB  - Springer
KW  - Wheat
KW  - AGROVOC
KW  - Height
KW  - Dwarfism
KW  - PCR
KW  - Genetic markers
N1  - Peer review
N2  - PCR-based markers were developed to detect the point mutations responsible for the two major semi-dwarfing genes Rht-B1b (Rht1) and Rht-D1b (Rht2) in wheat. These markers were validated by testing 19 wheat varieties of known Rht genotype. They included Rht-B1b and Rht-D1b dwarfs, double-mutant varieties and tall wheats. These were correctly genotyped with the Rht-B1b and Rht-D1b-specific primers, as well as markers specific for the tall alleles Rht-B1a and Rht-D1a. Using a family of doubled-haploid lines segregating for Rht-B1b and Rht-D1b, the markers were mapped to the expected homoeologous regions of chromosomes 4B and 4D, respectively. Both markers were strongly correlated with a reduction in height, accounting for 23% (Rht-B1b) and 44% (Rht-D1b) of the phenotypic variance in the population. These markers will have utility in marker-assisted selection of the Rht-B1b and Rht-D1b genes in wheat breeding programs
DO  - https://doi.org/10.1007/s00122-002-1048-4
T2  - Theoretical and Applied Genetics
ER  -