TY  - JA
AU  - Guzman,C.
AU  - Ortega,R.
AU  - Yamamori,M.
AU  - Peña-Bautista,R.J.
AU  - Alvarez,J.B.
TI  - Molecular characterization of two novel null waxy alleles in Mexican bread wheat landraces
PY  - 2015///
CY  - London (United Kingdom)
PB  - Elsevier
KW  - Wheat
KW  - AGROVOC
KW  - Food quality
KW  - Alleles
KW  - Landraces
N1  - Peer review
N2  - Starch is composed of two glucose polymers: amylose, formed by long linear chains, and amylopectin, which is larger and highly branched. The ratio between the two polymers (usually 22-35% amylose, 68-75% amylopectin) affects the starch properties, thereby determining different aspects of wheat quality. Waxy protein, which is the sole enzyme responsible for amylose synthesis, showed polymorphism in a collection of one hundred and three Mexican landraces, including null alleles at Wx-A1 and Wx-B1 loci, respectively. Molecular characterization of these alleles showed that the null Wx-A1 allele presented in only one accession was novel (Wx-A1o) and involved a deletion spanning the three and a half last exons of the gene. Some of the accessions lacking the Wx-B1 protein contained the common allele Wx-B1b (12.6%) (deletion of approximately 67 kb), while four others (3.8%) possessed the novel Wx-B1l, characterized by the deletion of one cytosine in the second exon of the gene and leading to a change in the ORF. This novel allele is particularly interesting because the absence of the Wx-B1 protein in this case was not associated with the lack of the other genes included in the approximately 67 kb region lost with the common null allele Wx-B1b
UR  - https://hdl.handle.net/20.500.12665/1327
DO  - https://doi.org/10.1016/j.jcs.2014.11.003
T2  - Journal of Cereal Science
ER  -