TY  - PRO
AU  - Hamza,S.
AU  - Abdennour,S.
AU  - Cherif,M.
AU  - Ginkel,M.van|McNab,A.|Krupinsky,J.
AU  - Harrabi,M.
AU  - Medini,M.
AU  - Rouassi,M.
AU  - Salah,A.B.
AU  - Sassi,T.
AU  - Strange,R.
ED  - Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico)
TI  - Characterization of Septoria tritici variants and PCR assay for detecting Stagonospora nodorum and Septoria tritici in wheat
SN  - 970-648-035-8
U1  - 632.4 
PY  - 1999///
CY  - Mexico, DF (Mexico)
PB  - CIMMYT
KW  - Disease resistance
KW  - AGROVOC
KW  - DNA
KW  - Pathogens
KW  - Plant diseases
KW  - Septoria
KW  - Stagonospora
KW  - Triticum
KW  - Wheat
KW  - CIMMYT
N2  - Pathogenic specialization of Septoria tritici was studied by inoculating 14 isolates of the fungus, of which seven were obtained from durum wheat and seven were isolated from bread wheat. Isolates obtained from durum wheat were more virulent on durum wheat, while those isolated from bread wheat were more severe on bread wheat, which revealed physiologic specialization of S. tritici to either bread or durum wheat. The sequence coding for the nuclear 5.8S rDNA and the internal transcribed spacer (ITS1 and ITS2) were amplified by polymerase chain reaction and sequenced for five isolates adapted to bread wheat and five isolates adapted to durum wheat. These sequences were identical between both variants, resulting in the absence of divergence inside tritici species. Septoria tritici and Stagonospora nodorum isolates collected from Tunisia were tested for amplification with specific primers to these pathogens. This revealed the primer’s efficiency to distinguish Septoria species and detect Stagonospora nodorum DNA with as little as 2 pg of DNA. Time course quantification of S. tritici mycelia after inoculation of resistant and susceptible cultivars distinguished those cultivars by the earliest date of apparition of a PCR product
ER  -