TY  - JA
AU  - Park,D.S.
AU  - Hahn,J.H.
AU  - Kim,H.G.
AU  - Kim,J.S.
AU  - Lim,C.K.
AU  - Shim,J.K.
AU  - Shrestha,R.
TI  - Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences
SN  - 0944-5013
PY  - 2009///
KW  - Bacterial spot
KW  - Detection
KW  - Pepper
KW  - Tomato
KW  - Xanthomonas campestris pv. vesicatoria
N2  - The present study describes PCR assay to detect bacterial spot caused by Xanthomonas campestris pv. vesicatoria in pepper and tomato. One set of PCR primer was developed to amplify gene required for an rhs family gene homologous to rhsA, cell envelope biogenesis, outer membrane. Only a PCR product of a 517 bp was produced in PCR reaction with the Xanthomonas campestris pv. vesicatoria (XCVF/XCVR) primer set. A specific, and highly sensitive and rapid PCR assay for the detection of X. campestris pv. vesicatoria was achieved. The protocol can be used as a reliable diagnostic tool for specific detection of X. campestris pv. vesicatoria in pepper or tomato
T2  - Microbiological Research
DO  - https://doi.org/10.1016/j.micres.2006.11.005
ER  -