TY  - JA
AU  - Gyawali,S.
AU  - Adhikari,T.B.
AU  - Burlakoti,R.R.
AU  - Neate,S.M.
AU  - Puri,K.D.
AU  - Shaobin Zhong
TI  - Genetic structure of Cochliobolus sativus populations sampled from roots and leaves of barley and wheat in North Dakota
PY  - 2012///
KW  - Amplified fragment length polymorphism
KW  - Barley
KW  - Cochliobolus sativus
KW  - Common root rot
KW  - Genetic differentiation
KW  - Spot blotch
KW  - Wheat
N1  - Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0931-1785
N2  - Common root rot (CRR) and spot blotch, caused by Cochliobolus sativus (Ito and Kurib.) Drechsl. ex Dast., are important diseases of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) worldwide. However, the population biology of C. sativus is still poorly understood. In this study, the genetic structure of three C. sativus populations, consisting of isolates sampled respectively from barley leaves (BL), barley roots (BR) and wheat roots (WR) in North Dakota, was analysed with amplified fragment length polymorphism (AFLP) markers. A total of 127 AFLP loci were generated among 208 C. sativus isolates analysed with three primer combinations. Gene diversity (H = 0.277?0.335) were high in all three populations. Genetic variation among C. sativus individuals within population accounted for 74%, whereas 26% of the genetic variation was explained among populations. Genetic differentiation was high (ØPT = 0.261, corrected = 0.39), whereas gene flow (Nm) ranged from 1.27 to 1.56 among the three populations analysed. The multilocus linkage disequilibrium (LD) (= 0.076?0.117) was moderate in C  sativus populations. Cluster analyses indicate that C. sativus populations differentiated according to the hosts (barley and wheat) and tissues (root and leaf) although generalists also exist in North Dakota. Crop breeding may benefit from combining genes for resistance against both specialists and generalists of C. sativus
DO  - https://doi.org/10.1111/j.1439-0434.2012.01956.x
T2  - Journal of Phytopathology
ER  -