TY - JA AU - Studholme,D.J. AU - Boonham,N. AU - Glover,R. TI - Application of high-throughput DNA sequencing in phytopathology SN - 0066-4286 PY - 2011/// KW - Bioinformatics KW - Diagnostics KW - Genomics KW - AGROVOC KW - Next-generation sequencing KW - Transcriptomics N1 - Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0066-4286 N2 - The new sequencing technologies are already making a big impact in academic research on medically important microbes and may soon revolutionize diagnostics, epidemiology, and infection control. Plant pathology also stands to gain from exploiting these opportunities. This manuscript reviews some applications of these high-throughput sequencing methods that are relevant to phytopathology, with emphasis on the associated computational and bioinformatics challenges and their solutions. Second-generation sequencing technologies have recently been exploited in genomics of both prokaryotic and eukaryotic plant pathogens. They are also proving to be useful in diagnostics, especially with respect to viruses T2 - Annual Review of PhytoPathology DO - https://doi.org/10.1146/annurev-phyto-072910-095408 ER -