TY  - JA
AU  - Feng Chen
AU  - Fuyan Zhang
AU  -  Morris,C.F.
AU  - He Zhonghu
AU  - Xianchun Xia
AU  - Dangqun Cui
TI  - Molecular characterization of the Puroindoline a-D1b allele and development of an STS marker in wheat (Triticum aestivum L.)
SN  - 1095-9963
PY  - 2010///
CY  - USA
PB  - Elsevier
KW  - Kernels
KW  - AGROVOC
KW  - Firmness
KW  - Wheat
KW  - Molecular characterization
KW  - Genetic markers
N1  - Peer review; Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210
N2  - Kernel hardness is mainly controlled by one major genetic locus on the short arm of chromosome 5D in bread wheat. Twelve Chinese and CIMMYT wheat cultivars were characterized for the deletion region of Pina-D1b genotype and developing a novel STS marker for this allele. PCR and SDS-PAGE were used to confirm the Pina-D1b genotype, and then 20 pairs of primers were designed to amplify the fragment including deletion region in Pina-D1b genotype by primer walking strategy. An STS marker Pina-N spanning deletion region in Pina-D1b was developed and sequencing results showed that all of 10 Pina- D1b genotypes uniformly possessed a 15,380 bp deletion in comparison with that of Chinese Spring with wild type. This study provided an alternative method to exam Pina-D1b by molecular marker and will accelerate identification of puroindoline alleles in bread wheat
UR  - https://hdl.handle.net/20.500.12665/613
DO  - https://doi.org/10.1016/j.jcs.2010.03.006
T2  - Journal of Cereal Science
ER  -