TY  - JA
AU  - Xinxia Sui
AU  - Lin-hai Wang
AU  - Xianchun Xia
AU  - Zhenlin Wang
AU  - He Zhonghu
TI  - Development of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay
SN  - 1836-0947
PY  - 2010///
CY  - Victoria (Australia)
PB  - CSIRO Publishing
KW  - Genetic markers
KW  - AGROVOC
KW  - Glutenins
KW  - PCR
KW  - Triticum aestivum
N1  - Peer review; Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1836-0947
N2  - Low-molecular-weight glutenin subunits (LMW-GS) have significant effects on the processing quality of end-use products of common wheat. It is more efficient to discriminate LMW-GS alleles with PCR-based molecular markers than with SDS-PAGE. In the present study, we developed an allele-specific PCR marker, designated Glu-B3abefg, which can be used to discriminate protein alleles Glu-B3a, b, e, f, and g simultaneously. Based on ten previously developed allele-specific STS markers, three multiplex PCRs, viz. Glu-B3c + Glu-B3d, Glu-B3b + Glu-B3g, and Glu-B3h + Glu-B3i, were established. Six Glu-B3 alleles (b, c, d, g, h, and i) could be discriminated using the three multiplex PCRs. Results of tests on 158 wheat varieties and lines using Glu-B3abefg and the three multiplex PCRs were consistent with those using the ten STS markers separately. The new allele-specific marker and three multiplex PCRs represent an efficient way to undertake marker-assisted selection of Glu-B3 alleles
UR  - https://hdl.handle.net/20.500.12665/792
T2  - Crop and Pasture Science
DO  - https://doi.org/10.1071/CP10241
ER  -