TY  - JA
AU  - BABU,R.
AU  - Chang-Jie Jiang
AU  - Xin Xu
AU  - Kottapalli,K.R.
AU  - Hiroshi Takatsuji
AU  - Akio Miyao
AU  - Hirohiko Hirochika
AU  - Shinji Kawasaki
TI  - Isolation, fine mapping and expression profiling of a lesion mimic genotype, splNF4050-8 that confers blast resistance in rice
SN  - 1432-2242
PY  - 2011///
CY  - Berlin (Germany)
PB  - Springer
KW  - AGROVOC
KW  - Rice
KW  - Chromosome mapping
KW  - Blasts (of plants)
KW  - Genetic resistance
N1  - Peer review; Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
N2  - We evaluated a large collection of Tos17 mutant panel lines for their reaction to three different races of Magnaporthe oryzae and identified a lesion mimic mutant, NF4050-8, that showed lesions similar to naturally occurring spl5 mutant and enhanced resistance to all the three blast races tested. Nested modified-AFLP using Tos17-specific primers and southern hybridization experiments of segregating individuals indicated that the lesion mimic phenotype in NF4050-8 is most likely due to a nucleotide change acquired during the culturing process and not due to Tos17 insertion per se. Inheritance and genetic analyses in two japonica × indica populations identified an overlapping genomic region of 13 cM on short arm of chromosome 7 that was linked with the lesion mimic phenotype. High-resolution genetic mapping using 950 F3 and 3,821 F4 plants of NF4050-8 × CO39 delimited a 35 kb region flanked by NBARC1 (5.262 Mb) and RM8262 (5.297 Mb), which contained 6 ORFs; 3 of them were ‘resistance gene related’ with typical NBS–LRR signatures. One of them harbored a NB–ARC domain, which had been previously demonstrated to be associated with cell death in animals. Microarray analysis of NF4050-8 revealed significant up-regulation of numerous defense/pathogenesis-related genes and down-regulation of heme peroxidase genes. Real-time PCR analysis of WRKY45 and PR1b genes suggested possible constitutive activation of a defense signaling pathway downstream of salicylic acid but independent of NH1 in these mutant lines of rice
UR  - https://hdl.handle.net/20.500.12665/294
T2  - Theoretical and Applied Genetics
DO  - https://doi.org/10.1007/s00122-010-1490-7
ER  -