TY  - JA
AU  - Dan Liang
AU  - Yang Fang-Ping
AU  - He Zhonghu
AU  - Yao Da-Nian
AU  - Xianchun Xia
TI  - Characterization of Lr34/Yr18, Rht-B1b, Rht-D1b genes in CIMMYT wheat cultivars and advanced lines using STS markers
SN  - 0578-1752
PY  - 2009///
CY  - Beijing (China)
PB  - Academy of Agricultural Sciences
KW  - Triticum aestivum
KW  - AGROVOC
KW  - Rusts
KW  - Disease resistance
KW  - Genes
KW  - Genetic markers
N1  - Peer review; Abstrac in Chinese and English; Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752
N2  - (Objective) Characterization of genes Lr34/Yr18, Rht-B1b (Rht1) and Rht-D1b (Rht2) in 263 CIMMYT wheat cultivars and advanced lines will benefit the improvement of rust resistance and plant height in Chinese wheat breeding program.  (Method) A total of 263 CIMMYT wheat cultivars and advanced lines were tested by STS markers to understand the distribution of resistance genes Lr34/Yr18 and dwarfing genes Rht-B1b and Rht-D1b. (Result) The marker csLV34 could amplify a 150 bp fragment in the lines with Lr34/Yr18, and a 229 bp fragment in those lines without Lr34/Yr18. Two complementary markers, NH-BF.2/WR1.2 and NH-BF.2/MR1, were used to investigate the alleles Rht-B1a and Rht-B1b, respectively. They could amplify a 400 bp fragment in the genotypes with Rht-B1a and Rht-B1b, respectively. The marker DF/MR2 could generate a 280 bp fragment in the genotype with Rht-D1b. Of the 263 lines, 57 generated a 150 bp fragment with the marker csLV34, indicating the presence of Lr34/Yr18 in these lines, with a frequency of 21.7%. Two hundred and sixteen lines (82.1%) were detected to have the allele Rht-B1b and 38 lines (14.4%) contained the allele Rht-D1b. Twenty-one lines were detected to possess both the wild-type alleles Rht-B1a and Rht-D1a, while 12 had two dwarfing genes Rht-B1b and Rht-D1b. (Conclusion) These STS markers could be useful to detect the genes Lr34/Yr18, Rht-B1b and Rht-D1b. The frequency of Rht-B1b was much higher than that of Rht-D1b in CIMMYT lines
UR  - https://hdl.handle.net/20.500.12665/619
DO  - https://doi.org/10.3864/j.issn.0578-1752.2009.01.003
T2  - Scientia Agricultura Sinica
ER  -