TY  - JA
AU  - Liu,L.
AU  - Aili Wang
AU  - Appels,R.
AU  - Junhong Ma
AU  - Xianchun Xia
AU  - Ping Lan
AU  - He Zhonghu
AU  - Bekes,F.
AU  - Yueming Yan
AU  - Wujun Ma
TI  - A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding
SN  - 0733-5210
PY  - 2009///
CY  - USA
PB  - Elsevier
KW  - Triticum aestivum
KW  - AGROVOC
KW  - Quality
KW  - Gel electrophoresis
KW  - Mass spectrometry
KW  - Glutenins
N1  - Peer review; Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210
N2  - High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7OE, 7OE+8, 7OE+8a*, 7OE+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7OE, 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki
UR  - https://hdl.handle.net/20.500.12665/1436
DO  - https://doi.org/10.1016/j.jcs.2009.05.006
T2  - Journal of Cereal Science
ER  -