TY  - JA
AU  - Jiro Murakami
AU  - Lewis,J.
AU  - Egusa,M.
AU  - Gargouri,S.
AU  - Motoichiro Kodama
AU  - Duveiller,E.
AU  - Ban Tomohiro
TI  - Evaluation of a real-time quantitative PCR assay for an effective screening of genotypes with FHB resistance and low mycotoxin accumulation in wheat and barley
SN  - 0133-3720
PY  - 2008///
CY  - Szeged (Hungary)
PB  - Cereal Research Non-Profit Company
KW  - AGROVOC
KW  - Fusarium
KW  - Vomitoxin
KW  - Gibberella zeae
KW  - PCR
N1  - Peer-review
N2  - To estimate fungal biomass in grains infected with Fusarium graminearum causing Fusarium Head Blight (FHB), a real-time PCR assay was applied for the quantification of the pathogen DNA based on the copy number of trichothecene synthesis gene (Tri5) sequence. Using 64 lines of wheat and barley in a field, we found significant correlations among the disease severity, deoxynivalenot (DON) accumulation and the pathogen DNA amount in the grains from each inoculated spike. The strong relationships were maintained in the susceptible population showing high DON accumulation but not the resistance population with low DON accumulation. These results indicate that the PCR assay could be useful discarding the germplasm very susceptible to FHB disease
T2  - Cereal Research Communications
ER  -