TY  - JA
AU  - Hongwei Geng
AU  - Zhang Qing-zhu
AU  - He Zhonghu
AU  - Lanqin Xia
AU  - Xinmin Chen
AU  - Desen Wang
AU  - Yanying Qu
TI  - Molecular characterization of transgenic wheats with ectopic puroindoline genes
SN  - 0578-1752
PY  - 2006///
CY  - Beijing (China)
PB  - Academy of Agricultural Sciences
KW  - Wheat
KW  - AGROVOC
KW  - Grain
KW  - Firmness
KW  - Indoles
KW  - Genes
KW  - Genetic transformation
KW  - Pollination
N1  - Peer review; Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752
N2  - 【Objective】Grain hardness is an important factor in determining wheat end-use quality and one of the most important targets in wheat quality improvement. 【Method】In this study, two monocotyledonous expression vectors pUBPa and pUBPb harboring pinA and pinB genes, the major genes controlling grain hardness, were transferred into wheat variety Zhongyou 9507 (pinB-D1b) via pollen-tube pathway, respectively, and transgenic plants were obtained. 【Result】The integration of the foreign puroindolines genes was confirmed by both PCR and genomic Southern blot analysis. Quantitative analysis of the friabilin on the surface of water-washed starch granules showed that the integration of foreign puroindolines genes indeed affected the expression level of puroindolines and grain texture. 【Conclusion】In conclusion, PINA and PINB (Puroindoline) interact to form friabilin and affect wheat grain texture.
UR  - https://hdl.handle.net/20.500.12665/721
T2  - Scientia Agricultura Sinica
ER  -