TY - REP AU - Murphy,J.F. AU - Clark,J.M.Jr AU - D'Arcy,C.J. ED - World Perspectives on Barley Yellow Dwarf International Workshop TI - Barley yellow dwarf virus RNA has a protein covalently linked to its 5' terminus SN - 968-6127-39 U1 - 91-013766 PY - 1990/// KW - Acids KW - Aphididae KW - Aphidoidea KW - Arthropoda KW - Carbohydrates KW - Disease transmission KW - Enzymes KW - Glycosides KW - Hemiptera KW - Homoptera KW - Insecta KW - Luteoviruses KW - Nucleic acids KW - Nucleic compounds KW - Organic acids KW - Pathogenesis KW - Pests of plants KW - Plant diseases KW - AGROVOC KW - Plant viruses KW - Proximate composition KW - Sternorrhyncha KW - Tobamoviruses KW - Viruses N1 - 10 ref N2 - The RNA of an Illinois isolate of barley yellow dwarf virus (BYDV) transmitted specifically by Rhopalosiphum padi (L.) was examined to determine the status of its 5' terminus. Initial studies were to determine if the 5' terminus of BYDV RNA was blocked, i.e., contained any derivatization other than phosphate(s). BYDV RNA, satellite tobacco necrosis virus RNA (STNV RNA), positive control) and tobacco mosaic virus RNA (TMV RNA, negative control) were treated with calf intestinal phosphatase, then T4 polynucleotide kinase and [y-32P] ATP were added. The 5' terminus of STNV RNA was consistently labeled with 32P, however, neither BYDV RNA nor TMV RNA T2 - Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Proceedings. Mexico, DF (Mexico). CIMMYT. 1990. p. 178-180 ER -