TY  - REP
AU  - Nazare Pereira,A.M.
AU  - Lister,R.M.
ED  - World Perspectives on Barley Yellow Dwarf International Workshop
TI  - Stability of ELISA activity in frozen samples of barley yellow dwarf virus-infected leaf and leaf extracts
SN  - 968-6127-39
U1  - 91-013910 
PY  - 1990///
KW  - Avena
KW  - Chemicophysical properties
KW  - Developmental stages
KW  - Freezants
KW  - Gramineae
KW  - Immunoenzyme techniques
KW  - Immunological techniques
KW  - Inorganic acid salts
KW  - Luteoviruses
KW  - Plant anatomy
KW  - AGROVOC
KW  - Plant diseases
KW  - Plant vegetative organs
KW  - Plant viruses
KW  - Processing aids
KW  - Salts
KW  - Viruses
N1  - 10 ref
N2  - When replicate samples of barley yellow dwarf virus-infected intact oat (Avena sativa L.) leaf, leaf extracts in phosphate buffer, or chloroform-clarified leaf extracts, were stored frozen at -20 deg C, -80 deg C, or in liquid nitrogen, their enzyme-linked immunosorbent assay (ELISA) activities fluctuated over time and according to the virus isolate involved. With the Purdue (P)-PAV isolate, no significant decrease of ELISA activity was noted with leaf, crude leaf extracts, or clarified extracts stored for up to 4 weeks at -20 deg C, -80 deg C, or in liquid nitrogen, except that clarified extracts stored at -20 deg C deteriorated after 1 week
T2  - Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Proceedings. Mexico, DF (Mexico). CIMMYT. 1990. p. 181-185
ER  -