TY - REP AU - Larkin,P.J. AU - Appels,R. AU - Banks,P. AU - Brettell,R.I.S. AU - Chen,X. AU - Cheng,Z.M. AU - Waterhouse,P.M. AU - Xin,Z.Y. AU - Zhou,G.H. ED - World Perspectives on Barley Yellow Dwarf International Workshop TI - Identification, characterization, and utilization of sources of resistance to barley yellow dwarf virus SN - 968-6127-39 U1 - 91-013943 PY - 1990/// KW - Acids KW - Asia KW - East asia KW - Gramineae KW - Hordeum KW - Immunoenzyme techniques KW - Immunological techniques KW - Luteoviruses KW - Nucleic acids KW - Nucleic compounds KW - Organic acids KW - Plant diseases KW - AGROVOC KW - Plant viruses KW - Resistance to injurious factors KW - Viruses KW - Triticum KW - Plant breeding N1 - 22 ref N2 - The enzyme-linked immunosorbent assay (ELISA) technique was used with a local isolate of the PAV serotype to screen wild grasses for resistance to barley yellow dwarf virus (BYDV). Thirteen species of grass showed resistance in that they could maintain low levels of the virus for one month after inoculation. The F1 hybrids with wheat (Triticum aestivum L.) showed good resistance. Particularly promising was a partial amphiploid from China called Zhong 4, which showed very low levels of PAV in ELISA. Probes with various ribosomal spacer sequences and 5S ribosomal sequences of DNA isolated from Zhong 4 suggested that some alien chromosomes present T2 - Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Proceedings. Mexico, DF (Mexico). CIMMYT. 1990. p. 415-420 ER -