TY  - JA
AU  - Richey,M.G.
AU  - Marek,E.T.
AU  - Schardl,C.L.
AU  - Smith,D.A.
TI  - Transformation of filamentous fungi with plasmid DNA by electroporation
SN  - 0031-949X
U1  - 90-024852 
PY  - 1989///
KW  - Acids
KW  - Cell structure
KW  - Cytoplasm
KW  - Cytoplasmic organelles
KW  - Energy
KW  - Energy sources
KW  - Fungi
KW  - AGROVOC
KW  - Moulds
KW  - Natural resources
KW  - Nucleic acids
KW  - Nucleic compounds
KW  - Organic acids
KW  - Organic compounds
KW  - Physics
KW  - Plant diseases
KW  - Plants
KW  - Protoplasm
KW  - Resources
KW  - Genetics
N1  - ill. references
N2  - We investigated the use of high-voltage electric pulse to transform Fusarium solani and Aspergillus nidulans with DNA. This method involved exposure of fungal protoplasts, in the presence of vector DNA, to a high-amplitude of exponential-decay electric pulse of short duration (up to 123 msec) delivered from a Gene Pulser apparatus. The effects of pulse amplitude and duration on transformation frequency and cell viability were evaluated. The optimal transformation is rapid, simple to perform, and avoids the use of toxic chemicals. It may also provide an alternative method of introducing foreign DNA into fungi that cannot be transformed by traditional methods
T2  - Phytopathology (USA). (Aug 1989). v. 79(8) p. 844-847
ER  -