TY  - JA
AU  - Sukhwinder-Singh
AU  - Dhaliwal,H.S.
AU  - Gill,B.S.
AU  - Gill,K.S.
AU  - Singh,H.
TI  - Towards molecular tagging of karnal but resistance gene(s) in wheat
SN  - 0974-1275
U1  - 95-033587 
PY  - 1994///
KW  - Bulk family analysis
KW  - Kamal bunt
KW  - RFLP
KW  - T. aestivum
KW  - Wheat
N1  - Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0971-7811
N2  - We describe preliminary results of cosegregation of a DNA probe with Karnal bunt resistance genets) in wheat. The tagging strategy utilized 4 uniformly resistant and 13 uniformly susceptible F3 families from a cross of Karnal bunt resistant (HD29) and susceptible (WL711) lines of Triticum aestivum. Bulked DNA of 8?10 plants in each family were tested by gel blot DNA hybridization. Thirty three per cent of the probes (11/33) detected restriction fragment length polymorphism (RFLP) between Karnal bunt resistant and susceptible lines. Probe Cxp1 from chromosome group 6 detected the resistant parent (HD29) polymorphic band in all the resistant family bulks but was missing in 6 uniformly susceptible family bulks. A similar pattern was observed with probe XKsu G34 from 1DL. The intensity of the resistant parent band in the susceptible family bulks was less as compared to the resistant family bulks. Probe Xksu H8 which mapped on chromosomes 2, 3, 5 and 7D on the T. tauschii map did not differentiate resistant family bulks from susceptible family bulks. Our results indicated that one or more resistance genets) are located on chromosome groups 1 and 6 of wheat
T2  - Journal of Plant Biochemistry and Biotechnology
DO  - https://doi.org/10.1007/BF03321955
ER  -