TY  - JA
AU  - D'Ovidio,R.
AU  - Porceddu,E.
AU  - Tanzarella,O.A.
TI  - Isolation of an alpha-type gliadin gene from Triticum durum Desf. and genetic polymorphism at the Gli-2 loci
SN  - 0394-9257
U1  - 93-095289 
PY  - 1992///
KW  - Acids
KW  - Cell structure
KW  - Chromosomes
KW  - Genetic engineering
KW  - Genetic variation
KW  - AGROVOC
KW  - Gramineae
KW  - Mathematical and statistical methods
KW  - Nucleic acids
KW  - Nucleic compounds
KW  - Nucleus
KW  - Organic acids
KW  - Polymorphism
KW  - Proteins
KW  - Triticum
KW  - Plant breeding
N1  - 1 table; 1 graph; 29 ref. Summary (En); Peer-review: No - Open Access: No
N2  - Genomic DNAs from Aegilops spp., Triticum spp., and related species were amplified by polymerase chain reaction (PCR). The oligonucleotides used as primers were terminal sequDEes of the coding region of an alpha-type gliadin gene. The PCR amplification produced an uniform band in all analysed Triticum and Aegilops genotypes, except Ae. speltoides. The PCR products of durum wheat cultivar Lira and T. urartu were cloned. Southern analysis of nulli-tetrasomic lines, carried out with digoxigenin labeled probe, demonstrated that the PCR amplification products represent alpha-type gliadin sequDEes. N-terminal sequDEe comparison of the T
T2  - Journal of Genetics and Breeding
ER  -