TY  - JA
AU  - Immonen,A.S.T.
TI  - Comparison of callus culture with embryo culture at different times of embryo rescue for primary triticale production
SN  - 1573-5060
PY  - 1993///
KW  - Callus
KW  - Embryo culture
KW  - Secale cereale
KW  - Tissue culture
KW  - Triticales
KW  - Wheat
KW  - AGROVOC
N1  - Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0014-2336
N2  - Callus culture of immature wheat-rye hybrid embryos was compared with embryo culture in 2 experiments. Embryos were rescued from field grown female plants at 2 day intervals 13-21 days after pollination and (1) cultured on MS medium supplemented with 2 mg 2,4-D/litre followed by plant regeneration on hormone free MS medium with half strength mineral salts, and (2) cultured on Taira and Larter medium. Observations were made on embryo size and condition at time of rescue (experiment 1) and embryo development directly into plants (embryo culture) or through somatic embryogenesis (callus culture). Fewer 19 and 21-day-old embryos developed into plants from callus culture than from embryo culture in experiment 1. Callus culture was more efficient than embryo culture in promoting plant recovery from 17-day-old embryos in experiment 2. The number of plants per embryo was significantly higher from callus culture than from embryo culture. In both experiments callus culture promoted embryogenesis in more embryos than developed in embryo culture. Embryo rescue 15-17 days after pollination was optimal in both experiments
T2  - Euphytica
ER  -