TY - JA AU - Bhatnagar,D. AU - Hill,R.A. AU - Lee,L.S. AU - McCormick,S.P. TI - Identification of O-methylsterigmatocystin as an aflatoxin B1 and G1 precursor in Aspergillus parasiticus SN - 1098-5336 PY - 1987/// KW - Aflatoxins KW - Aspergillus KW - Biosynthesis KW - Metabolites KW - Mycelium KW - Plant diseases KW - AGROVOC KW - Plant pathology N1 - Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0099-2240; Tables, graphs, literture cited p. 1032-1033 N2 - An isolate of A. parasiticus CP461 (SRRC 2043) produced no detectable aflatoxins, but accumulated O-methylsterigmatocystin (OMST). When sterigmatocystin (ST) was fed to this isolate in a low-sugar medium, there was an increase in the accumulation of OMST, without aflatoxin synthesis. When radiolabelled [14C]OMST was fed to resting mycelia of a non-aflatoxin-, non-ST-,and non-OMST-producing mutant of A. parasiticus AVN-1 (SRRC 163), 14C-labelled aflatoxins B1 and G1 were produced; 10 nmol of OMST produced 7.8 nmol of B1 and 1 nmol of G1, while 10 nmol of ST produced 6.4 nmol of B1 and 0.6 nmol of G1. A time course study of aflatoxin synthesis in ST feeding T2 - Applied and Environmental Microbiology ER -