TY  - JA
AU  - Kirihara,J.A.
AU  - Hunsperger,J.P.
AU  - Mahoney,W.C.
AU  - Messing,J.W.
TI  - Differential expression of a gene for a methionine-rich storage protein in maize
PY  - 1988///
KW  - Methionine
KW  - Prolamines
KW  - Seed
KW  - Seeds
KW  - Zea mays
KW  - AGROVOC
N1  - Illustrations, tables, references p. 483-484
N2  - On the basis of the sequence of the N-terminal 30 amino acids of a 10 kD methionine-rich zein protein, 2 mixed oligonucleotides were synthesized and usedto probe a maize endosperm cDNA library. A full-length cDNA clone encoding the 10 kD zein was isolated;its nucleotide sequence predicted a polypeptide of 129 amino acids, preceded by a signal peptide of 21 amino acids. The predicted polypeptide was unique in its extremely high content of methionine (22.5%). The inbred line BSSS53, which has increased seed methionine, was compared to W23, a standard inbred line. Northern blot analysis showed that the relative RNA levelsfor the 10 kD zein were enhanced
T2  - Molecular and General Genetics
ER  -