TY  - JA
AU  - Lizardi,P.M.
AU  - Guerra,C.E.
AU  - Kramer,F.R.
AU  - Lomeli,H.
AU  - Tussie-Luna,I.
TI  - Exponential amplification of recombinant-RNA hybridization probes
PY  - 1988///
KW  - Biotechnology
KW  - Plasmodium
KW  - RNA
N1  - Illustrations, graphs, references p. 1202
N2  - Recombinant RNA molecules were synthesized that function both as hybridization probes and as templates for exponential amplification by Qbeta replicase. Each recombinant consists of a sequence specific for Plasmodium falciparum, embedded within the sequence of MDV-1 RNA, which is a natural template for Qbeta replicase. The probe sequence was inserted within a hairpin loop that occurs on the exterior of MDV-1 RNA. The recombinant RNAs hybridize specifically to complementary DNA, despite topological constraints on the probe domain, are replicated at the same rate as MDV-1 RNA, despite their additional length, and are able to serve as templates for the
T2  - BioTechnology
ER  -