TY  - JA
AU  - Matsubara,A.
AU  - Hattori,M.
AU  - Kawano,S.
AU  - Kojima,M.
AU  - Narita,M.
AU  - Shikata,E.
AU  - Uyeda,I.
TI  - Purification and serology of a Japanese isolate of barley yellow dwarf virus
PY  - 1985///
KW  - Barley
KW  - Barley yellow dwarf virus
KW  - Elisa
KW  - Immunology
KW  - Japan
KW  - Purification
KW  - Rhopalosiphum
N1  - Illustrations, tables, literature cited p. 157
N2  - BYDV was purified from infected barley plants by an improved procedure whichyielded 44 æg virus/100 g tissues. The isolate had isometric particles, diam. 27.3 nm and a UV absorbance spectrum with an av. A260/A280 ratio of 1.71. In ELISA using the double antibody sandwich method, the virus reacted with homologous antiserum but not with antiserum to potato leafroll virus and vice versa. It reacted slightly with heterologous antisera to other luteoviruses suchas PLRV, soyabean dwarf and beet western yellows viruses in agar gel double diffusion tests
T2  - Annals of the Phytopathological Society of Japan
ER  -