TY  - JA
AU  - Shewry,P.R.
AU  - Bunce,N.A.C.
AU  - Forde,B.G.
AU  - Kreis,M.
TI  - Polymorphism at the hor 1 locus of barley (Hordeum vulgare L.)
PY  - 1985///
KW  - Barley
KW  - DNA
KW  - Enzymes
KW  - Genetic maps
KW  - Genomes
KW  - AGROVOC
KW  - Peptides
KW  - Polymorphism
KW  - Prolamines
KW  - Proteins
N1  - Illustrations, tables, references p. 403-404
N2  - Two-dimensional electrophoretic analysis of C-hordein fractions from 6 cultivars with different alleles at the Hor1 locus (Hor1Pr, Ar, Ke, Ju, Ho and So) showed extensive polymorphism. A total of 34 major polypeptides were identified, with 4-18 present in each cultivar. For each cultivar, 6-10 restriction fragments of genomic DNA hybridized to a cDNA clone related to C- hordein;the total number of restriction fragments was 22, and most pairs of cultivars had more restriction fragments than polypeptides in common. There wereestimated to be 20-30 C-hordein genes per haploid genome. It is concluded that cultivars differ mainly in the extent of gene and
T2  - Biochemical Genetics
ER  -