TY  - JA
AU  - Lyznik,L.A.
AU  - Chang,K.L.
AU  - Grimes,H.D.
AU  - Hodges,T.K.
AU  - Kamo,K.K.
AU  - Ryan,R
TI  - Stable transformation of maize:  The impact of feeder cells on protoplast growth and transformation efficiency
PY  - 1989///
KW  - Antibiotics
KW  - Cell culture
KW  - Genes
KW  - Genetic transformation
KW  - Protoplasts
KW  - Zea mays
KW  - AGROVOC
N1  - Illustrations, tables, graphs, references p. 295
N2  - Protoplast cultures of the F1 hybrid A188 X Black Mexican Sweet were transformed using 2 plasmids, pPUR carrying genes coding for beta-glucuronidase (GUS) and pKAN carryong the gene neo neomycin resistance, by either electroporation or PEG [polyethylene glycol] treatment. Total GUS activity, measured 2 days after transformation, was 5-fold higher in protoplasts cultured on feeder cells compared to those grown in the absence of feeder cells. Since the specific activity of GUS was only slightly higher in the transformed protoplasts plated over feeder cells, the stimulation in transient gene expression resulted mainly from the improved environment
T2  - Plant Cell Reports
ER  -