Cloning of cereal stress genes. A molecular approach to plant improvement for adaptation to environmental changes
Material type:
TextLanguage: En Publication details: 1988Description: p. 67-73Subject(s):
In:
Cereal breeding related to integrated cereal production609902Summary: The in vitro translation of mRNAs isolated from stressed and unstressed barley plants showed that the appearance of new proteins in response to heat shock was a consequence of (1) induction and translation of new mRNAs during thestress and (2) modification in the aggregation state of the proteins or in some of their chemico physical properties. The mRNAs corresponding to the induced proteins were cloned as cDNA either in phages or plasmids and the cDNA library analysed by differential hybridization with mRNA from stressed and unstressed plants. This analysis led to the identification of cDNAs coding for heat shock proteins. The analysis
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Reprint | CIMMYT Knowledge Center: John Woolston Library | Reprints Collection | REP-4365 (Browse shelf(Opens below)) | 1 | Available | 609902 |
Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: Reprints Collection Close shelf browser (Hides shelf browser)
The in vitro translation of mRNAs isolated from stressed and unstressed barley plants showed that the appearance of new proteins in response to heat shock was a consequence of (1) induction and translation of new mRNAs during thestress and (2) modification in the aggregation state of the proteins or in some of their chemico physical properties. The mRNAs corresponding to the induced proteins were cloned as cDNA either in phages or plasmids and the cDNA library analysed by differential hybridization with mRNA from stressed and unstressed plants. This analysis led to the identification of cDNAs coding for heat shock proteins. The analysis
English
Reprints Collection
Click on an image to view it in the image viewer