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Illusions in visual assessment while determining the genetic basis of tolerance in maize to maize streak virus

By: Kyetere, D.T | Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico).
Contributor(s): Ransom, J.K.|Palmer, A.F.E.|Zambezi, B.T.|Mduruma, Z.O.|Waddington, S.R.|Pixley, K.V.|Jewell, D.C [eds.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Addis Ababa (Ethiopia) CIMMYT : 1997ISBN: 92-9146-025-7.Subject(s): Disease control | Disease resistance | Genetic variation | Plant diseases | CIMMYT | Zea mays AGROVOC | Maize streak geminivirus AGROVOC | Hybrids AGROVOC | Plant breeding AGROVOCDDC classification: 633.15 Summary: Eighty seven tropical maize recombinant inbred lines (RILs), F1 and their two parents were evaluated for tolerance to maize streak geminivirus (MSV), using controlled leafhopper infestation at two locations. One glasshouse experiment was conducted at Namulonge, Uganda, and two field experiments at the Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Harare, Zimbabwe. A rating scale of 1 (tolerant) to 5 (susceptible) based on streaked areas on leaf surfaces was used. Analysis of variance of MSV data showed significant differences among RILs. Transgressive segregation for higher tolerance was observed for some RILs. The performance of the parents, F,, and RILs was consistent across the two locations. The F1's reaction was intermediate between the parents in the Namulonge experiment and similar to the tolerant parent in the two Harare experiments. This suggests a degree of dominance is associated with MSV tolerance. Genetic analysis of the RILs using molecular genetic markers, restriction fragment length polymorphisms (RFLPs), was carried out. The association between these markers and MSV tolerance was determined using single factor analysis of variance (SFAOV) and two interval mapping procedures. Results from the three approaches identified the same genomic region accounting for the variation in MSV tolerance.Collection: CIMMYT Publications Collection
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Publications Collection 633.15 EAS No. 5 (Browse shelf) 1 Available 1W624172
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Eighty seven tropical maize recombinant inbred lines (RILs), F1 and their two parents were evaluated for tolerance to maize streak geminivirus (MSV), using controlled leafhopper infestation at two locations. One glasshouse experiment was conducted at Namulonge, Uganda, and two field experiments at the Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Harare, Zimbabwe. A rating scale of 1 (tolerant) to 5 (susceptible) based on streaked areas on leaf surfaces was used. Analysis of variance of MSV data showed significant differences among RILs. Transgressive segregation for higher tolerance was observed for some RILs. The performance of the parents, F,, and RILs was consistent across the two locations. The F1's reaction was intermediate between the parents in the Namulonge experiment and similar to the tolerant parent in the two Harare experiments. This suggests a degree of dominance is associated with MSV tolerance. Genetic analysis of the RILs using molecular genetic markers, restriction fragment length polymorphisms (RFLPs), was carried out. The association between these markers and MSV tolerance was determined using single factor analysis of variance (SFAOV) and two interval mapping procedures. Results from the three approaches identified the same genomic region accounting for the variation in MSV tolerance.

English

9801|AGRIS 9702

Jose Juan Caballero

CIMMYT Publications Collection

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