Molecular analysis of resistance to Pyrenophora tritici-repentis, race 1 in PBW343/Kenya Nyangumi population
Material type:
TextPublication details: 2013Description: p. 172Summary: Tan spot, caused by an ascomycete Pyrenophora tritici-repentis, causes 5-15% yield losses in certain environments and adversely affects the quality of grain. With increasing adoption of conservation agricultural practices and global warming, tan spot incidence and severity has been increasing over decades in various wheat growing areas. The objectives of this study were to decipher the genetics and map the resistance to tan spot in the PBW343/Kenya Nyangumi population. A population of 169 F6 recombinant inbred lines (RILs) was evaluated for disease reaction at the seedling stage under greenhouse conditions. Tan spot was induced by P. tritici-repentis, race 1 isolate Ptr-1. Two weeks old seedlings were spore inoculated and rated eight days later on a 1 to 5 lesion type disease scale. Diversity Array Technology (DArTs) and simple sequence repeats (SSRs) markers were used to map the resistance. Segregation pattern of the RILs indicated that resistance is quantitative for tan spot reaction. Several genomic regions contributing to tan spot resistance were observed including the long arm of chromosome 1B harboring the pleiotropic gene Lr46/Yr29/Pm39. This is first report of the gene Lr46/Yr29/Pm39 contributing to tan spot resistance.
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-7565 (Browse shelf(Opens below)) | Available |
Abstract only
Tan spot, caused by an ascomycete Pyrenophora tritici-repentis, causes 5-15% yield losses in certain environments and adversely affects the quality of grain. With increasing adoption of conservation agricultural practices and global warming, tan spot incidence and severity has been increasing over decades in various wheat growing areas. The objectives of this study were to decipher the genetics and map the resistance to tan spot in the PBW343/Kenya Nyangumi population. A population of 169 F6 recombinant inbred lines (RILs) was evaluated for disease reaction at the seedling stage under greenhouse conditions. Tan spot was induced by P. tritici-repentis, race 1 isolate Ptr-1. Two weeks old seedlings were spore inoculated and rated eight days later on a 1 to 5 lesion type disease scale. Diversity Array Technology (DArTs) and simple sequence repeats (SSRs) markers were used to map the resistance. Segregation pattern of the RILs indicated that resistance is quantitative for tan spot reaction. Several genomic regions contributing to tan spot resistance were observed including the long arm of chromosome 1B harboring the pleiotropic gene Lr46/Yr29/Pm39. This is first report of the gene Lr46/Yr29/Pm39 contributing to tan spot resistance.
Global Wheat Program|Genetic Resources Program
English
Lucia Segura
INT2868|INT3098|INT0610
CIMMYT Staff Publications Collection