Abstract only

Fusarium head blight (FHB), caused by Fusarium graminearum, is a serious disease in wheat (Triticum aestivum L.) causing mycotoxin contamination of deoxynivalenol (DON). Since Chinese wheat cultivar Sumai 3 was extensively used as a FHB resistant source in the world wide breeding programs, it has been observed that the possible genetic and phenotypic variation among some of the Sumai 3 accessions used in different countries as a derivative. The objective of this work is to identify phenotypic variation of FHB resistance and genetic diversity among the Sumai 3 derivatives. DNA fingerprinting by SSR markers was applied to identify genetic variation among those Sumai 3 accessions. Austrian (Sumai 3-AUT) and Japanese (Sumai 3-JPN) accessions showed polymorphism of specific chromosome regions compared to the original Chinese accession. Field evaluation at CIMMYT in Mexico identified the significant variation for DON accumulation in the grains among Sumai 3-AUT and JPN but not for FHB incidence and severity on the spikes of them. Subsequent field evaluation at NARO/KARC in Japan characterized lower level for FHB incidence and DON accumulation in Sumai 3-AUT rather than Sumai 3-JPN. Multiple field experiments indicated that reducing DON accumulation in Sumai 3-AUT was similar under different environment condition. Genetic diversity by DArT marker showed the possibility of reducing DON accumulation in Sumai 3-AUT. DNA markers to distinguish haplotype of the previous QTL reports explained that QTL on 5AS and 2DS would be responsible for the variation of DON accumulation not on 3BS and 6B in Sumai 3-AUT. Sumai 3-AUT was identified as possessing resistant allele of TaMRP-D1, a candidate gene of the 2DS QTL, suggested that TaMRP-D1 supposed to contribute lowering DON level. This work is the first indication that clarified the variation among Sumai 3 collections for FHB field response with genetic diversit

English

Lucia Segura

CIMMYT Staff Publications Collection