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Identification of stem rust resistance genes in hexaploid wheat and their introgression using molecular markers

By: Contributor(s): Material type: TextTextLanguage: English Publication details: 2012. Beijing (China) : BGRI,Description: 1 pageISBN:
  • 978-0-615-70429-6
Subject(s): In: Proceedings Borlaug Global Rust Initiative Technical Workshop; Poster Abstracts. Theme 2: New Sources of Rust Resistance; Beijing (China); 1-4 Sep. 2012 p. 113Summary: The occurrence of Pgt race Ug99 in Iran was a reminder to the Indian sub-continent of the urgent need for crop protection in order to ensure food security for people whose diets constitute 40% wheat. Improving resistance in an already widely grown variety could restrict the spread of Ug99 in the short term. Three wheat cultivars, HUW234, HUW468 and HUW510, and five potential resistance donors were tested for presence of Sr25, SrR and SrWeb using molecular markers. Seedlings of the 3 recipient and 5 potential donors were tested for seedling responses to Pgt races 62G29 (40A), 62G29-1 (40-1) and 62G13-3 (40-2) at Shimla. Among the five donors, PMBWIR 4 (PRL/2*PASTOR//PBW343*2/KUKUNA/3/TACUPETO F2001*2/KUKUNA) appeared to carry all three resistance genes and was therefore used as the resistance donor to introgress the genes into the widely grown cv. HUW234. BC2F4 progenies were selected by foreground selection followed by background selection using 63 genome-wide SSR markers. The population was also screened against stem rust under field conditions using the prevalent Pgt race 21A-2. Five plants were identified as having all the three resistance genes and showing >90% similarity with the recipient parent. Lines derived from these plants are currently undergoing agronomic assessment.
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Abstract only

The occurrence of Pgt race Ug99 in Iran was a reminder to the Indian sub-continent of the urgent need for crop protection in order to ensure food security for people whose diets constitute 40% wheat. Improving resistance in an already widely grown variety could restrict the spread of Ug99 in the short term. Three wheat cultivars, HUW234, HUW468 and HUW510, and five potential resistance donors were tested for presence of Sr25, SrR and SrWeb using molecular markers. Seedlings of the 3 recipient and 5 potential donors were tested for seedling responses to Pgt races 62G29 (40A), 62G29-1 (40-1) and 62G13-3 (40-2) at Shimla. Among the five donors, PMBWIR 4 (PRL/2*PASTOR//PBW343*2/KUKUNA/3/TACUPETO F2001*2/KUKUNA) appeared to carry all three resistance genes and was therefore used as the resistance donor to introgress the genes into the widely grown cv. HUW234. BC2F4 progenies were selected by foreground selection followed by background selection using 63 genome-wide SSR markers. The population was also screened against stem rust under field conditions using the prevalent Pgt race 21A-2. Five plants were identified as having all the three resistance genes and showing >90% similarity with the recipient parent. Lines derived from these plants are currently undergoing agronomic assessment.

Global Wheat Program

Text in English

INT2917

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