Knowledge Center Catalog

The genetic diversity of the DRF1 gene in durum wheat and its ancestors

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT : 2011Description: p. 109ISBN:
  • 978-970-648-179-5
Summary: The DRF1 (dehydration responsive factor 1) gene belongs to DREB-family and is involved in the abiotic stress response. The same alternative splicing mechanism characterizing the DRF1 gene expression in wheat was also observed in its wild relatives. The genetic diversity of DRF1 gene has been investigated in durum wheat, Triticum urartu, Aegilops speltoides and in other Aegilops. The DRF1 gene was characterized in around 100 durum accessions, more than 200 wild ancestor accessions and in a RIL durum wheat population consisting of 177 inbred lines. SNP, SSR and insertions/deletions were identified in view of gaining more knowledge about the intra- and inter-specific variability of the DRF1 gene in its different forms and homeologous copies. A PCR selective test based on the gene sequence was set up and used for clustering the RIL population.
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection CIS-6543 (Browse shelf(Opens below)) Available
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Abstract only

The DRF1 (dehydration responsive factor 1) gene belongs to DREB-family and is involved in the abiotic stress response. The same alternative splicing mechanism characterizing the DRF1 gene expression in wheat was also observed in its wild relatives. The genetic diversity of DRF1 gene has been investigated in durum wheat, Triticum urartu, Aegilops speltoides and in other Aegilops. The DRF1 gene was characterized in around 100 durum accessions, more than 200 wild ancestor accessions and in a RIL durum wheat population consisting of 177 inbred lines. SNP, SSR and insertions/deletions were identified in view of gaining more knowledge about the intra- and inter-specific variability of the DRF1 gene in its different forms and homeologous copies. A PCR selective test based on the gene sequence was set up and used for clustering the RIL population.

Global Wheat Program

English

Lucia Segura

INT2585

CIMMYT Staff Publications Collection


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