Abstract only

To face the demands for increasing global food production, plant breeding is adopting various approaches to develop improved cultivars and increase crop yields. Molecular marker technology offers a wide range of approaches improving selection strategies in breeding. Marker assisted selection (MAS), marker assisted backcrossing and genomic selection have shown to provide valuable assistance to increase selection efficiency through indirect selection for agronomically valuable traits and are therefore increasingly adapted in breeding programs. The discovery of a large number of single nucleotide polymorphisms (SNPs) in crops has accelerated the development of genotyping technology with increased throughput and low cost. However, most platforms are qualified for the screening of large numbers of markers and genotypes simultaneously while in most wheat breeding programs MAS is currently applied using a limited number of markers across large segregating populations during narrow time intervals. For successful incorporation in wheat breeding programs, flexible and multi-parallel genotyping detection methods would be profitable. We have used available and novel sequence information to develop SNP and Indel detection primers using the KASPar technology (KBioscience) linked to genes that are extensively applied in MAS programs in wheat at CIMMYT and worldwide. SNP assays of known polymorphism for Lr34, VPM, Fhb1, Glu-D1, GPC-B1, Rht-B1, Rht-D1 are presented. SNP assays were validated on a set of wheat lines to assess their suitability for high-throughput MAS. The SNP assays have found to be been robust, reliability and significantly reduce the time and cost which may accelerate the transfer of markers to practical plant breeding

Global Wheat Program

English

Lucia Segura

INT2692

CIMMYT Staff Publications Collection