Quantitative trait loci for adult plant resistance to wheat stem rust in cultivar K-Nyangumi
Material type:
TextPublication details: 2011Description: p. 187Online resources: Summary: The challenge posed by Ug99 and other Puccinia graminis f. sp tritici races has necessitated faster discovery of effective sources of resistance to stem rust and their efficient use through marker assisted selection. The Kenyan bread wheat cultivar K-Nyangumi exhibits adult plant resistance in the field, but is susceptible at the seedling stage. To dissect this resistance, the cross K-Nyangumi/PBW343 was made and 223 F6 RILs generated by single seed descent were inoculated and evaluated for adult plant stem rust resistance for two seasons at Njoro-Kenya in 2010. RILs were phenotyped for leaf rust resistance at Cd. Obregon, Mexico during 2009-2010 season. Results indicated continuous variation implicating quantitative resistance. Inclusive composite interval mapping (ICIM) (http://www. isbreeding.net) software was used to analyze the QTL maps with 599 polymorphic DArT markers. Preliminary analysis revealed three chromosomes - 1B, 3B and 6A - significantly associated with stem rust resistance and explaining 5%, 23% and 5% of the phenotypic variation, respectively. These results paved the way for current fine mapping of the significant genomic regions. The identified markers are candidates for MAS.
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-6338 (Browse shelf(Opens below)) | Available |
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Abstract only
The challenge posed by Ug99 and other Puccinia graminis f. sp tritici races has necessitated faster discovery of effective sources of resistance to stem rust and their efficient use through marker assisted selection. The Kenyan bread wheat cultivar K-Nyangumi exhibits adult plant resistance in the field, but is susceptible at the seedling stage. To dissect this resistance, the cross K-Nyangumi/PBW343 was made and 223 F6 RILs generated by single seed descent were inoculated and evaluated for adult plant stem rust resistance for two seasons at Njoro-Kenya in 2010. RILs were phenotyped for leaf rust resistance at Cd. Obregon, Mexico during 2009-2010 season. Results indicated continuous variation implicating quantitative resistance. Inclusive composite interval mapping (ICIM) (http://www. isbreeding.net) software was used to analyze the QTL maps with 599 polymorphic DArT markers. Preliminary analysis revealed three chromosomes - 1B, 3B and 6A - significantly associated with stem rust resistance and explaining 5%, 23% and 5% of the phenotypic variation, respectively. These results paved the way for current fine mapping of the significant genomic regions. The identified markers are candidates for MAS.
Genetic Resources Program|Global Wheat Program
English
Lucia Segura
INT2843|INT2833|INT0610|INT3098
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