Investigating the role of SrCad and Sr2 on stem rust race TTKST in wheat
Material type:
TextPublication details: 2011Description: p. 186Online resources: Summary: Stem rust race TTKST with virulence to Sr24 and Sr31 is a threat to wheat production globally. AC Cadillac expresses resistance to TTKST. AC Cadillac has Sr2 and SrCad, whereas Carberry has neither. Both lines are thought to have other unidentified Sr genes, and both have Lr34 which reportedly works synergistically with SrCad, where SrCad stem rust resistance is better in the presence of Lr34. The objective of this study was to use a doubled haploid (DH) population derived from Carberry/AC Cadillac to study the effect of Sr2 and SrCad in controlling TTKST. The parents and 219 DH lines were evaluated to TTKST in a stem rust nursery near Njoro, Kenya, for adult plant stage resistance while seedling reactions against TTKSK were evaluated in a bio-containment facility (Morden, Canada). Stem rust severity and pustule type were recorded. Parents and DH lines were evaluated with molecular markers linked to Sr2 (Xgwm533 and X3B028F08) and SrCad (Xcfd49). In the DH population, Xgwm533 and X3B028F08 mapped 3 cM apart. Single factor analysis of variance indicated that Xcfd49 was significantly associated with seedling resistance as well as adult plant resistance in the 2009 and 2010 Kenya nurseries. The Sr2-linked markers were significantly associated with resistance associated with pustule type in 2010. No significant SrCad by Sr2 interaction was observed. Results suggested that SrCad provided good resistance to race TTKST in an Lr34 background and simultaneous presence of Sr2 with SrCad did not confer significantly better resistance than SrCad alone.
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-6337 (Browse shelf(Opens below)) | Available |
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Abstract only
Stem rust race TTKST with virulence to Sr24 and Sr31 is a threat to wheat production globally. AC Cadillac expresses resistance to TTKST. AC Cadillac has Sr2 and SrCad, whereas Carberry has neither. Both lines are thought to have other unidentified Sr genes, and both have Lr34 which reportedly works synergistically with SrCad, where SrCad stem rust resistance is better in the presence of Lr34. The objective of this study was to use a doubled haploid (DH) population derived from Carberry/AC Cadillac to study the effect of Sr2 and SrCad in controlling TTKST. The parents and 219 DH lines were evaluated to TTKST in a stem rust nursery near Njoro, Kenya, for adult plant stage resistance while seedling reactions against TTKSK were evaluated in a bio-containment facility (Morden, Canada). Stem rust severity and pustule type were recorded. Parents and DH lines were evaluated with molecular markers linked to Sr2 (Xgwm533 and X3B028F08) and SrCad (Xcfd49). In the DH population, Xgwm533 and X3B028F08 mapped 3 cM apart. Single factor analysis of variance indicated that Xcfd49 was significantly associated with seedling resistance as well as adult plant resistance in the 2009 and 2010 Kenya nurseries. The Sr2-linked markers were significantly associated with resistance associated with pustule type in 2010. No significant SrCad by Sr2 interaction was observed. Results suggested that SrCad provided good resistance to race TTKST in an Lr34 background and simultaneous presence of Sr2 with SrCad did not confer significantly better resistance than SrCad alone.
Global Wheat Program
English
Lucia Segura
INT2843|I1705031
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