Mapping and exploiting valuable resistant genes/QTLs for the main fungal pathogens affecting durum wheat
Material type:
TextPublication details: Beijing (China) Chinese Academy of Agricultural Sciences : 2010Description: p. 55Summary: Durum wheat (Triticum durum Desf.) is a main cereal crop for the Mediterranean area, where various cereal diseases can cause epidemics and important production losses: Septoria tritici blotch, leaf rust and powdery mildew are common in Southern Europe and North Africa. The identification of effective sources of resistance, their genetic mapping and the development of molecular markers suitable for marker-assisted selection are major objectives of durum wheat improvement. In collaboration with CIMMYT and molecular plant pathologists, a program for mapping useful genetic variation through a joint linkage and association mapping approach has been undertaken at DiSTA. Up to now, three RIL populations (Kofa × Svevo, Meridiano × Claudio and Colosseo × Lloyd) each including ca. 180 RILs and a germplasm panel of 210 cultivated accessions suitable for genome-wide association mapping (Maccaferri et al. 2005) have been profiled with SSR and DArT markers and phenotyped at the seedling and adult plant stages (artificially inoculated field trials). Examples of the use of these materials for QTL dissection studies include: a major gene for leaf rust resistance on chr. 7BL (Colosseo × Lloyd), two major QTLs for the powdery mildew infection response (Meridiano × Claudio) on chrs. 6BL and 7BL, QTLs for slow rusting (Kofa × Svevo) and chromosomal regions associated to Septoria infection response. Association mapping with the germplasm panel has provided additional information on the genetics of the resistances. Fine mapping of some of the major genes/QTLs identified from this mapping effort is underway with the aims of developing markers tightly linked to the genes/QTLs and eventually cloning of the genes. The rice-wheat synteny conservation provides the bases for SNP development based on the rice-wheat orthologs and candidate gene identification. A number of markers associated to the resistance loci are being used by PSB in their breeding programs for the release of improved durum cvs. An overview of the main results will be presented.
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-6271 (Browse shelf(Opens below)) | Available |
Abstract only
Durum wheat (Triticum durum Desf.) is a main cereal crop for the Mediterranean area, where various cereal diseases can cause epidemics and important production losses: Septoria tritici blotch, leaf rust and powdery mildew are common in Southern Europe and North Africa. The identification of effective sources of resistance, their genetic mapping and the development of molecular markers suitable for marker-assisted selection are major objectives of durum wheat improvement. In collaboration with CIMMYT and molecular plant pathologists, a program for mapping useful genetic variation through a joint linkage and association mapping approach has been undertaken at DiSTA. Up to now, three RIL populations (Kofa × Svevo, Meridiano × Claudio and Colosseo × Lloyd) each including ca. 180 RILs and a germplasm panel of 210 cultivated accessions suitable for genome-wide association mapping (Maccaferri et al. 2005) have been profiled with SSR and DArT markers and phenotyped at the seedling and adult plant stages (artificially inoculated field trials). Examples of the use of these materials for QTL dissection studies include: a major gene for leaf rust resistance on chr. 7BL (Colosseo × Lloyd), two major QTLs for the powdery mildew infection response (Meridiano × Claudio) on chrs. 6BL and 7BL, QTLs for slow rusting (Kofa × Svevo) and chromosomal regions associated to Septoria infection response. Association mapping with the germplasm panel has provided additional information on the genetics of the resistances. Fine mapping of some of the major genes/QTLs identified from this mapping effort is underway with the aims of developing markers tightly linked to the genes/QTLs and eventually cloning of the genes. The rice-wheat synteny conservation provides the bases for SNP development based on the rice-wheat orthologs and candidate gene identification. A number of markers associated to the resistance loci are being used by PSB in their breeding programs for the release of improved durum cvs. An overview of the main results will be presented.
Global Wheat Program
English
Lucia Segura
INT2585
CIMMYT Staff Publications Collection
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