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Forming double cross hybrids using two line synthetics

By: Contributor(s): Material type: TextTextLanguage: English Publication details: 2010. Long Beach, CA (USA) :Description: 1 pageSubject(s): In: ASA-CSSA-SSSA 2010 International Annual Meeting: Green revolution 2.0: Food+Energy and Environmental SecuritySummary: In maize breeding programs in the developing world, double cross hybrids remain of interest due to lower seed costs. However, their production is cumbersome for small seed companies, due to the need to maintain four parental inbreds and to manage the F1 parent crossing blocks as well as the double-cross seed production field. One way to simplify double cross production is to form hybrids from F2 bulks or two-line synthetics derived from each single cross parent. In such a scheme, single cross parents would be advanced to F2 and maintained as two-line synthetics, then crossed to form the certified seed. Allelic frequencies in gametes produced by an F2 or a two-line synthetic should be similar to those produced by an equivalent F1, and therefore the array of genotypes formed from an F2 x F2 or two line synthetic cross should be equivalent to that produced by a conventional F1 x F1 cross. Both methods should therefore generate hybrids with similar performance. We tested this hypothesis in the CIMMYT tropical lowland maize breeding program in 2009. Five conventional double cross hybrids (F1 x F1) and corresponding F2 x F2 crosses were compared in trials at five locations in Southern Mexico. There was no significant difference in yield between F1 x F1 and F2 x F2 crosses. Further trials are being conducted to confirm these findings in 2010 in eastern and southern Africa. Although seed yields on F2 or two-line synthetic parents will be slightly less than on F1 seed parents , the method should be of interest to small seed companies in Africa who often have difficulty managing inbred lines, and could help small, local seed companies currently producing only open-pollinated varieties to embark on hybrid seed production. This, in turn, will increase the availability of low-cost hybrid maize seed to African smallholders.
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Abstract only

In maize breeding programs in the developing world, double cross hybrids remain of interest due to lower seed costs. However, their production is cumbersome for small seed companies, due to the need to maintain four parental inbreds and to manage the F1 parent crossing blocks as well as the double-cross seed production field. One way to simplify double cross production is to form hybrids from F2 bulks or two-line synthetics derived from each single cross parent. In such a scheme, single cross parents would be advanced to F2 and maintained as two-line synthetics, then crossed to form the certified seed. Allelic frequencies in gametes produced by an F2 or a two-line synthetic should be similar to those produced by an equivalent F1, and therefore the array of genotypes formed from an F2 x F2 or two line synthetic cross should be equivalent to that produced by a conventional F1 x F1 cross. Both methods should therefore generate hybrids with similar performance. We tested this hypothesis in the CIMMYT tropical lowland maize breeding program in 2009. Five conventional double cross hybrids (F1 x F1) and corresponding F2 x F2 crosses were compared in trials at five locations in Southern Mexico. There was no significant difference in yield between F1 x F1 and F2 x F2 crosses. Further trials are being conducted to confirm these findings in 2010 in eastern and southern Africa. Although seed yields on F2 or two-line synthetic parents will be slightly less than on F1 seed parents , the method should be of interest to small seed companies in Africa who often have difficulty managing inbred lines, and could help small, local seed companies currently producing only open-pollinated varieties to embark on hybrid seed production. This, in turn, will increase the availability of low-cost hybrid maize seed to African smallholders.

Global Maize Program

Text in English

INT2825|INT2396|INT2714

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